Bioluminescence Assays and Reagents
BPS Bioscience Inc ARE Reporter Kit (Nrf2 Antioxidant Pathway), 500 reactions
The ARE Reporter kit is designed for monitoring the activity of the Nrf2 antioxidant pathway in cultured cells. The kit contains a transfection-ready ARE luciferase reporter vector, which is an Nrf2 pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ARE responsive elements located upstream of a minimal promoter. The ARE reporter is premixed with a constitutively expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. This negative control is critical to determining pathway-specific effects and background luciferase activity.
Abcam WAY 262611, Wnt pathway activator, 25MG
MW 318.4 Da, Purity >97%. Wnt pathway activator. Selectively inhibits Dkk1 with no GSK-3β inhibition. TCF-Luciferase agonist (EC₅₀ = 0.63 μM). Increases bone formation rate in vivo. Orally active.
The product is subject to the following: Abcam Restricted Use Statement
BPS Bioscience Inc ARE Luciferase Reporter HepG2 Cell Line, part number 60513, firefly luciferase, adherent cell line, >1 x 10^6 cells in 1 ml Cell Freezing Medium, validated for Nrf2 pathway response
The BPS Bioscience ARE Luciferase Reporter HepG2 Cell Line (Nrf2 Antioxidant Pathway) is specifically engineered to enable precise monitoring of the Nrf2 antioxidant response pathway. This stable, adherent HepG2 cell line incorporates a firefly luciferase reporter gene under the control of an Antioxidant Response Element (ARE), facilitating the direct correlation between luciferase expression and Nrf2 activation. Its reliable performance has been validated through stimulation with various compounds known to influence the antioxidant pathway.
- Stable, adherent cell line
- Features firefly luciferase as the reporter gene
- Monitors Nrf2 antioxidant response pathway activity
- Validated with tert-butylhydroquinone, sulforaphane, RTA-408
- Absence of mycoplasma confirmed
- Suitable for research applications
- Stored in liquid nitrogen upon receipt
- Biosafety Level: BSL-1
Invivogen QUANTI-Luc™ 4 Lucia/Gaussia, Highly Sensitive, Reliable Bioluminescence Detection, 500 ml
QUANTI-Luc™ 4 Lucia/Gaussia is an advanced bioluminescence detection reagent that enables precise quantitative measurement of Lucia and Gaussia luciferase reporter gene expression. This ready-to-use solution ensures high sensitivity and reproducibility with exceptional signal-to-noise ratios, making it ideal for gene expression studies and reporter assays. Its unique formulation minimizes background interference and improves detection reliability, setting it apart in the field of molecular biology research.